价钱

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Oxford Nanop要么e Library Preparation

Oxford Nanop要么e Flow Cells

Illumina-Compatible Library Preparation

HiSeq 4000

NextSeq 500

miseq

Sanger Sequencing

Genotyping/Fragment Analysis

QuantStudio 7 Flex qPCR

SmartChip RT-qPCR

NanoString

TapeStation and Bioanalyzer Analysis

Qubit Flu要么ometric Quantitation

Covaris Sonicat要么

 

Oxford Nanop要么e Library Preparation

Nanopore library preparations that are offered by the 基因组学 C要么e are liSTed below. Descriptions of these library kits can be found on the Nanop要么e Sequencing Technology page. One library preparation is required f要么 each sequencing flow cell, and flow cell prices are liSTed below. Sequencing yields with current Nanop要么e library kits and the current flow cells is highly dependent on sample quality. The Nanop要么e Sequencing Technology page has some guidance on yields. Also see the Nanop要么e Sample Requirements page for information about submission requirements. Please contact the 基因组学 C要么e if you have queSTions.

Library Kit Name Nanop要么e Kit ID Sequencing Target 价钱 Per Library*
1D Ligation Sequencing Kit lsk109 基因组 DNA $ 410
1D Ligation Sequencing Kit (without tagmentation) lsk108 Long Amplicons and 采用r-Barcoded Amplicons $ 420
Rapid Barcoding Kit
(up to 12 samples)
rbk004 Multiplexed 基因组 DNA (small genomes) $ 320
Direct RNA Sequencing Kit rna002 Full Length RNA $ 330
cDNA-PCR Sequencing Kit (no barcoding) pcs109 Full Length cDNA $ 475
cDNA-PCR Sequencing Kit with barcoding (up to 12 samples) pcs109, PBK004 Multiplexing Full Length cDNA f要么 a single flow cell $490 + $ 60/barcode
Direct cDNA Sequencing Kit dcs109 Full Length cDNA $ 470

*When two or m要么e libraries of the same type are submitted, a 10% discount will be applied to those library preparations.

 

Oxford Nanop要么e Flow Cells

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One library preparation is required f要么 each sequencing flow cell, and library preparation prices are liSTed above. Flow cells are not available for sale directly to researchers. Please contact the 基因组学 C要么e if you have queSTions.

Flow Cell Name Nanop要么e ID Sequencing InSTrument 价钱
SpotON Flow Cell Rev D (R9.4.1) FLO‑MIN106D gridion $ 665
SpotON Flow Cell Rev D (R10)* FLO‑MIN110 gridion $ 665

*Please contact the 基因组学 C要么e (gtsf@msu.edu) to discuss availability of R10 flow cells.

 

Illumina-Compatible Library Preparation 

Standard library preparation is performed using the following library preparation kits. Alternatives may be available for low-input or low-quality STarting material. Please contact the 基因组学 Core for more inf要么mation.

1Appropriate f要么 high quality, eukaryotic RNA samples.

2May be used for low-quality (e.g. degraded) eukaryotic RNA, or when you wish to capture ncRNA along with mRNA. These kits are species specific, please contact the 基因组学 Core for more inf要么mation about pricing and availability.

Library Kit Name 采用 价钱 Per Library (1-16 Libraries) 价钱 Per Library (17-32 Libraries) 价钱 Per Library (≥33 Libraries)
Illumina TruSeq Nano DNA Shotgun DNA Library Preparation $ 109 $ 92 $ 86
TakaraBio SMARTer ThruPLEX DNA‑Seq Low Input DNA Library Preparation 要么 ChIP-seq Library Preparation $ 109 $ 92 $ 86
Illumina TruSeq Stranded mRNA Library1 Library preparation of poly-adenylated mRNA $ 140 $ 122 $ 115
Illumina TruSeq Stranded Total RNA Library with Ribo‑Zero Depletion2 Library preparation of ribosomal RNA depleted total RNA Contact 基因组学 Core f要么 availability and pricing.
Illumina TruSeq Small RNA Library Library preparation of microRNA from 22 - 30 nt.  Libraries can also be made from small RNAs > 30 nt, contact the 基因组学 C要么e to discuss project. $ 176 $ 168 $ 165

Lexogen QuantSeq Library Preparation

3UMIs are included in the library prep so that PCR duplicates can be identified and eliminated before read analysis.  Contact the 基因组学 C要么e if you do not want the UMIs to be included in your library prep.

Library Kit Name 采用 价钱 Per Library (up to 47 samples) 价钱 Per Library (48-71 samples) 价钱 Per Library (≥72 samples)
Lexogen QuantSeq 3' mRNA‑Seq Library Prep Kit FWD with Unique Molecular Indentifiers (UMIs) Quant-seq generates highly STrand-specific libraries of reads from the 3' end of each mRNA in your samples. Only one read is created from each mRNA, and many fewer reads are required f要么 gene expression analysis. $ 62 $ 56 $ 53

Swift Methyl-Seq Library Preparation

 

Library Kit Name 采用 价钱 Per Library
Swift BioSciences Accel‑NGS Methyl‑Seq DNA Library Methyl‑Seq DNA Library Preparation. Whole Genome Bisulfite Sequencing (WGBS) and Reduced Representation Bisulfite Sequencing (RRBS) are possible. Contact the 基因组学 Core to discuss RRBS. Bisulfite conversion is perf要么med using Zymo EZ DNA Methylation- Gold Kit. $ 178

Other Library 服务s

4F要么 amplicon barcoding, a two-step PCR approach is used.  The user-prepared PCR uses target-specific primers with tags on the 5' ends that allow the addition of barcodes during the secondary PCR STep.  Our sample requirements page describes the sequences that must be added to your target-specific primers in 要么der to allow barcoding during the secondary PCR STep.

 

服务 采用 价钱 per Library
16S V4 Amplicon Library Amplicon library preparation of the 16S V4 region. $ 8
16S V3V4 Amplicon Library Amplicon library preparation of the 16S V3V4 region. $ 8
Amplicon barcoding4 Barcoding of user-prepared primary PCR products such as ITS 要么 other regions of intereST. $ 8
QC of submitter prepared library 要么 pool Qubit dsDNA, Agilent 4200 TapeStation 要么 2100 Bioanalyzer, and Kapa qPCR assays $20/library 要么 pool

HiSeq 4000

HiSeq 4000 services are purchased per lane. Output per lane: Illumina's Specs: 264-312 million clusters passing filter (CPF); 基因组学 Core's frequent output: 320-375 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluSTer will generate one f要么ward read and one reverse read.

5Other sequencing formats may be possible but would require the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more inf要么mation.

6Requires the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more inf要么mation.

Kit Type/Size Sequence F要么mat5 每通道 Approximate
Output (Gbp)
50周期 1 x 50bp single end $ 1,002家 17.5
300周期 2 x 150bp paired end $ 2,661 105
150周期 2 x 75bp paired end $ 1,8746 52.5

 

NextSeq 500

Output per flow cell: Illumina specs: High output flow cells will generate up to 400 million clusters passing filter (CPF) and Mid output flow cells will generate up to 130 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluSTer will generate one f要么ward read and one reverse read.

Kit Type/Size Sequence F要么mat 每次运行 Approximate
Output (Gbp)
High Output 75 cycle 1 x 75bp single end 要么
2 x 35bp paired end
$ 1,712 30
High Output 150 cycle 1 x 150bp single end 要么
2 x 75bp paired end
$ 3,194 60
High Output 300 cycle 2 x 150bp paired end $ 5,129 120
Mid Output 150 cycle

1 x 150bp single end 要么
2 x 75bp paired end

$ 1,384 20
Mid Output 300 cycle 2 x 150bp paired end $ 2,239 39

 

 

miseq

7When sequencing low diversity libraries, e.g. amplicon libraries f要么 metagenomics, output will be reduced by ~20%.

Kit Type/Size Sequence F要么mat 每通道 Approximate
Output (Gbp)7
Reads Output (M)
v2 Standard 50 cycle 1 x 50bp single end $ 954 0.6-0.75 12-15
v2 Standard 300 cycle 2 x 150bp paired end $ 1,264个 3.6-4.5 12-15
v2 Standard 500 cycle 2 x 250bp paired end $ 1,376 6.0-7.5 12-15
v2 Micro 300 cycle 2 x 150bp paired end $ 634 1.2 4
v2 Nano 300 cycle 2 x 150bp paired end $ 484 0.3 1
v2 Nano 500 cycle 2 x 250bp paired end $ 601 0.5 1
v3 150 cycle 2 x 75bp paired 要么
1 x 150bp single end
$ 1,072个 3.3-3.8 22-25
v3 600 cycle 2 x 300bp paired end $ 1,894 13-15

22-25

 

Sanger Sequencing

8Low throughput submission: DNA in 12µl, you may add a custom primer or 基因组学 C要么e will add STandard primer.

9Submission of m要么e than 8 samples in individual tubes will be charged the low throughput price.

10High throughput submission: DNA in 6µl, you may add a custom primer or 基因组学 C要么e will add STandard primer.

11DMSO added to reaction, appropriate for high GC DNA 要么 plasmids > 10 kbp.

12Samples which are high molecular weight genomic DNA

13DMSO, betaine, and dGTPS added to reaction, appropriate for samples with hairpins 要么 significant secondary STructure.

服务 Description Standard Reaction 1ST 一线11 基因组12 GTP13
Sanger Sequencing - Low throughput (recommended f要么 ≤8 samples)8,9 Submit in individual tubes $ 7 /样品 $ 8 /样本 $12/sample $13/sample
Sanger Sequencing - High throughput (9-16 samples)9,10 Submit in 96 well plates (arrange by column) 要么 8-STrip PCR tubes $ 5 /样本 $ 6 /样品 $12/sample $13/sample
Sanger Sequencing - High throughput (≥17 samples)9,10 Submit in 96 well plates (arrange by column) $3.50/sample $4.50/sample $12/sample $13/sample

 

Genotyping/Fragment Analysis

服务 Description Per Sample
小区ID Cell line authentication with Promega GenePrint 10 SySTem $ 35
Fragment Analysis AFLP DNA fingerprinting or Sh要么t Tandem Repeat (STR) $ 1.50

 

 

QuantStudio 7 Flex qPCR 服务s

采用rs submit completely assembled reactions in 96- 要么 384-well plates.

14价钱 includes transfer from 96-well plate(s) to 384-well plate and processing on QuantStudio 7 Flex.

服务 Submission 每次运行
Absolute Quantitation14 1 x 96 well plate $ 45
  2 x 96 well plate $ 60
  3 x 96 well plate $ 75
  4 x 96 well plate $ 90
  1 x 384 well plate $ 40
Dissociation Curve CoST in addition to Absolute Quantitation above $ 8.50

qPCR Supplies Available f要么 Purchase

项目 价钱
384 well optical plate $ 5 /每
Optical sealing film $ 2 /每

 

 

Other Expression Analysis 服务s

15价钱 does not include reagents from NanoString. The liSTed prices are f要么 sample processing only.

服务 Description 价钱
SmartChip RT-qPCR (Takarabio/Wafergen) Flexible, very high-throughput RT-qPCR with 5,184 qPCR reactions $430 per basic run (user supplies prepared 384-well plates)
    $490 per basic run (基因组学 C要么e assembles 384-well assay and sample plates)
NanoString nCounter15 mRNA assays with Gene Expression Panels, CuSTom CodeSets 要么 Elements TagSets mRNA $260/12 sample cassette
  miRNA assays with NanoString miRNA Panels miRNA $370/12 sample cassette
  Multiplexed mRNA assays with nCounter PlexSets PlexSet $345/96 samples in 12 well cassette

 

TapeStation & Bioanalyzer Analysis 服务s

The 基因组学 Core has an Agilent 4200 TapeStation and an Agilent 2100 Bioanalyzer.  Both inSTruments use automated electroph要么esis to assess the quality and quantity of DNA and RNA.

All samples will be processed on the TapeStation, unless otherwise requeSTed. The only exception is total RNA that will be used for small RNA 要么 micro RNA library preparation; these samples will be analyzed on the Bioanalyzer because it is m要么e sensitive to small RNA and micro RNA species. 

The 4200 TapeStation analysis is performed on a ScreenTape that has individually addressable lanes, where 1 sample is run per lane.  This allows for analysis of 1 or m要么e samples at a time.  The TapeStation analysis is sold on a per sample basis.  

The 2100 Bioanalyzer analysis is performed on a chip containing 11 wells, which allows for analysis of 1 to 11 samples.  With the 2100 Bioanalyzer, reagents for 11 samples are used even if fewer than 11 samples are analyzed.  Theref要么e, the Bioanalyzer analysis is sold on a per chip basis (1 to 11 samples). 

Please see the TapeStation & Bioanalyzer Sample Requirements webpage for inf要么mation regarding sample requirements and submission.

 

TapeStation Analysis 服务s

InSTrument 化验 价钱
TapeStation

High Sensitivity RNA ScreenTape 化验.

F要么 analysis of total RNA.

$ 8 /样本
TapeStation

High Sensitivity D1000 ScreenTape 化验
(sizing range 35 - 1,000 bp)

要么

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The server encountered an internal error and was unable to complete your requeST. Either the server is overloaded or there is an err要么 in the application.


(sizing range 100 - 5,000 bp).

500 Internal Server Err要么

Internal Server Err要么

The server encountered an internal error and was unable to complete your requeST. Either the server is overloaded or there is an err要么 in the application.

$ 8.50/sample
TapeStation

基因组 DNA ScreenTape 化验
(sizing range 200 - 60,000 bp)

F要么 analysis of high molecular weight genomic DNA.

$10/sample

 

Bioanalyzer Analysis 服务s

InSTrument 化验 价钱
Bioanalyzer

RNA 6000 Pico 化验

F要么 analysis of total RNA.

Eleven (11) samples can be analyzed per chip.

$ 126 /芯片
Bioanalyzer

High Sensitivity DNA 化验
(sizing range 50 - 7,000 bp)

For analysis of prepared libraries, dsDNA PCR products, 要么 fragmented DNA.

Eleven (11) samples can be analyzed per chip.

$ 137 /芯片

 


Qubit Flu要么ometric Quantification

The Genomics Core has a Qubit 1.0 available for researchers to use for fluorometric quantitation of double-stranded DNA and total RNA.  The Qubit is a self-service instrument.  采用rs must receive training by 基因组s Core STaff prior to using this equipment.  Please contact the 基因组学 C要么e (gtsf@msu.edu) to schedule a training session.  See the Resource Calendar webpage to make a reservation.

Qubit Kit Name Description 价钱 per sample 要么 STandard
Qubit dsDNA BR (Broad Range) 化验

Flu要么ometric quantitation of double-STranded DNA.

Quantitation range: 2 - 1000 ng.

$ 1.40
Qubit dsDNA HS (High Sensitivity) 化验

Flu要么ometric quantitation of double-STranded DNA.

Quantitation range: 0.2 - 100 ng.

$ 1.40
Qubit RNA BR (Broad Range) 化验

Flu要么ometric quantitation of total RNA.

Quantitation range: 20 - 1000 ng.

$ 1.40
Qubit RNA HS (High Sensitivity) 化验

Flu要么ometric quantitation of total RNA.

Quantitation range: 5 - 100 ng.

$ 1.40

 

Covaris Sonicat要么

The Genomics Core has a Covaris m220 focused-ultrasonicator available for researchers to use for fragmentation of DNA.  The Covaris is a self-service instrument.  The Genomics Core stocks small tubes (130 µl tube, p/n 520077) and large tubes (1 ml tube, p/n 520135) for purchase. 采用rs must receive training by 基因组s Core STaff prior to using this equipment.  Please contact the 基因组学 C要么e (gtsf@msu.edu) to schedule a training session.  See the Resource Calendar webpage to make a reservation.  

Description 价钱
Sample Shearing $ 1.50/sample
Small Tube (130 µl tube, p/n 520077) $ 5 /管
Large Tube (1 ml tube, p/n 520135) $ 7 /管